MicroRNA-controlled quantitative trait loci (QTLs)

See: Nutrient-dependent/pheromone-controlled adaptive evolution: a model (6/14/13)

A good model predicts.  In my refutation of the neo-Darwinian pseudoscientific nonsense touted in Mutation-Driven Evolution (6/14/13), I predicted:

The role of the microRNA/messenger RNA balance… in adaptive evolution will certainly be discussed in published works that will follow.

On 6/13/13 25691 of the currently existing 87961 articles that mention microRNA had been published.

For comparison 8134 of the currently existing 13145 articles that mention QTL had been published.  Because nearly 5-fold more articles that mention microRNA were published in 6 years compared to those that mention QTL, I watched my predictions about polycombic adaptive evolution overtake claims about the virus-driven mutation-driven hecatombic evolution of diseases.

See also:

QTLs: Your introduction to VirusGate (1)  (6/12/19)

QTLs: Your introduction to VirusGate (2) (6/13/19)

QTLs: Your introduction to VirusGate (3) (6/18/19)

Six years after the prediction in my model about “The role of the microRNA/messenger RNA balance…” the model can be examined in the context of this accurate representation of biophysically constrained viral latency.

MicroRNAs meet with quantitative trait loci: Small powerful players in regulating quantitative yield traits in rice (6/17/19)

…a number of quantitative trait loci (QTL) controlling yield‐related traits have been identified. Some of them are actually controlled by miRNAs…

Light activated microRNA biogenesis controls the creation of miRNAs that link the control of QTLs to yield‐related agronomic traits:

image

A summary of the miRNAs (highlighted in light blue) and their target genes (highlighted in purple) controlling rice yield‐related agronomic traits, including grain number, grain weight, tiller number, fertility, and leaf inclination. The miRNAs marked in red color are those controlling just one of the above mentioned five traits, based on the recent publications. The arrow and nail shape indicate positive and negative regulation, respectively.

The fact that microRNAs control QTL-linked traits/yield‐related agronomic traits became obvious when serious scientists learned that the creation of sunlight had been linked from the creation of ATP to the creation of RNA and RNA interference via the light-activated fixation of carbon, which is required for the creation of the microRNAs.

From 1964 to 2018, some geneticists may have toyed with the idea of accurately representing top-down cause and effect by linking the claims in McEwen et al., (1964) to the claims in the patent for RNA-guided human genome engineering.

See: Dependence of RNA synthesis in isolated thymus nuclei on glycolysis, oxidative carbohydrate catabolism and a type of “oxidative phosphorylation”

The synthesis of RNA in isolated thymus nuclei is ATP dependent.

That requirement links light-activated carbon fixation from the creation of the microRNA-RNA-peptide nanocomplex to biophysically constrained viral latency across all kingdoms.

Finally, the claim about light-activated carbon fixation rose to the surface in What’s in the Water at the Church Lab? A Conversation Between George Church and Jorge Conde (2/14/18)

At 15:10 in the Edge video and audio, George Church claims

“…the cyanobacteria turn out that they fix light ah as well or better than land plants…” before he claims “We found the enzymes that occur in nature, which was not obvious…”

It was obvious to all serious scientists that the energy-dependent creation of one or more enzymes would be required to link light-activated carbon fixation to the creation of the microRNA-RNA-peptide nanocomplex. In the 2015 patent application for RNA-Guided Human Genome Engineering, George Church et al., made this claim:

5. Repetitive elements or endogenous viral elements can be targeted with engineered Cas+gRNA systems in microbes, plants, animals, or human cells to reduce deleterious transposition or to aid in sequencing or other analytic genomic/transcriptomic/proteomic/diagnostic tools (in which nearly identical copies can be problematic).

He linked the light-activated fixation of carbon from the creation of the microRNA-RNA-peptide nanocomplex to biophysically constrained viral latency via RNA interference in microbes, plants, animals, and human cells. Because energy-dependent changes in amino acids must be linked to the creation of different enzymes, George Church qualified himself to lead the VirusGate investigation, or be the first defendant.

In either role, he is brilliant.

On 5/18/19, I asked him:

How do non-homologous end joining (NHEJ knockouts) for repair of double-strand breaks in DNA compare to naturally occurring food energy-dependent RNA-mediated alternative splicing?

He responded within the hour:

NHEJ mutations enable testing of “full nulls”, which are difficult to achieve with RNA-splicing, RNAi, CRISPRi, etc.

RNA interference is the link from NHEJ testing of the “full nulls” to the energy-dependent biophysically constrained alternative splicing of pre-mRNA that links viral latency to protection of all organized genomes from the virus-driven degradation of their messenger RNA. The pre-mRNAs are now called microRNAs and the fact that microRNAs control quantitative trait loci eliminates the pseudoscientific nonsense of mathematical models. The mathematical models failed to link the creation of sunlight to plant growth and the physiology of reproduction, which is required for the RNA-mediated survival of all living genera.

See also: Physiology is rocking the foundations of evolutionary biology (4/12/13)

Perhaps the elegant mathematics and the extraordinary reputation of the scientists involved blinded us to what now seems obvious: the organism should never have been relegated to the role of mere carrier of its genes.

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Author: James Kohl

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