11/21/18 MicroRNAs as therapeutic agents
11/22/18 The tipping point (revisited): 80,000 publications
11/24/18 at 8:14 AM from Nature Research (Nature@newsletter.nature.com)
To:jvkohl@bellsouth.net
“Optogenetics is a method that uses light to modulate molecular events in a targeted manner in living cells or organisms. Research by leading labs in the field, including those of Breakthrough Prize winners, is presented in this free-to-access collection of articles from Scientific Reports.”
Epigenetic Editing of Ascl1 Gene in Neural Stem Cells by Optogenetics 2/9/17
Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics 1/6/17
Optogenetic silencing of nociceptive primary afferents reduces evoked and ongoing bladder pain 11/20/17
Biophotonic energy as information and biophysically constrained optogenetic RNA interference link light-activatated microRNA biogenesis to microRNA-mediated prevention and/or effective treatment of all pathology. The virus-driven degradation of messenger RNA must be prevented by food energy the context of biophysically constrained transgenerational epigenetic inheritance.
Nature Research began to fall decades behind after McEwen et al., (1964) linked the creation of ATP to the creation of RNA.
The synthesis of RNA in isolated thymus nuclei is ATP dependent.
UV-Induced Charge Transfer States in DNA Promote Sequence Selective Self-Repair 1/13/16
…photoexcited DNA leads to reactive charge transfer states.
More likely it is the driving power represented by the oxidation potential of the radical ion, which controls the repair. Thus, repair capacities are strongly sequence-dependent, creating DNA regions with different tendencies of self-repair. This self-healing activity represents the simplest sequence-dependent DNA repair system.
Energy as information and constrained endogenous RNA interference 2/15/17
Feedback loops link quantized energy as information to biophysically constrained RNA-mediated protein folding chemistry. Light induced energy-dependent changes link angstroms to ecosystems from classical physics to chemistry/chirality and to molecular epigenetics/autophagy. The National Microbiome Initiative links microbial quorum sensing to the physiology of reproduction via endogenous RNA interference and chromosomal rearrangements.
The rearrangements link energy-dependent fixed amino acid substitutions to the Precision Medicine Initiative via genome wide inferences of natural selection. This detailed representation of energy-dependent natural selection for codon optimality links biologically- based cause and effect from G protein-coupled receptors to RNA-mediated amino acid substitutions and the functional structure of supercoiled DNA.
Energy-dependent polycombic ecological adaptations are manifested in supercoiled DNA. Chromosomal inheritance links the adaptations from morphological phenotypes to healthy longevity via behavioral phenotypes. For contrast, virus-driven energy theft is the link from messenger RNA degradation to negative supercoiling, constraint breaking mutations, and hecatombic evolution. The viral hecatomb links transgenerational epigenetic inheritance from archaea to Zika virus-damaged DNA, which typically is repaired by endogenous RNA interference and fixation of RNA-mediated amino acid substitutions in organized genomes.
James V. Kohl detailedl how quantized energy-dependent microRNA biogenesis links the pheromone-controlled physiology of reproduction to biophysically constrained viral latency and healthy longevity. Links from what organisms eat to the enzyme-dependent metabolism of food also link metabolic networks to microRNA-mediated genetic networks in the context of RNA interference. Drug therapies alter RNA interference by altering cell type differentiation in all cells of all individuals of all species. For comparison, during the past 26 years, Kohl has detailed how the chemistry of protein folding is biophysically constrained at every level of biological organization by conserved molecular mechanisms.